|Application ||WB, E|
|Calculated MW||56594 Da|
|Antigen Region||109-138 aa|
|Other Names||NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER, NAB2, MADER|
|Target/Specificity||This NAB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human NAB2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NAB2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).|
|Cellular Location||Nucleus. Note=Isoform 2 is not localized to the nucleus.|
|Tissue Location||Widely expressed at low levels. Highly expressed in melanoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
NAB2 is a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by this protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex.
Srinivasan,R., J. Biol. Chem. 281 (22), 15129-15137 (2006)
Kumbrink,J., J. Biol. Chem. 280 (52), 42785-42793 (2005)
Lucerna,M., J. Biol. Chem. 278 (13), 11433-11440 (2003)
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