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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RYK Antibody   

RYK Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RYK Antibody AP7677a
    Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175)(Cat. #AP7677a). AP7677a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - RYK Antibody AP7677a
    Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175)(Cat. #AP7677a). AP7677a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - RYK Antibody AP7677a
    Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175)(Cat. #AP7677a). AP7677a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - RYK Antibody AP7677a
    Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175)(Cat. #AP7677a). AP7677a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - RYK Antibody AP7677a
    The anti-RYK Pab (Cat. #AP7677a) is used in Western blot to detect RYK in Jurkat cell lysate.
  • WB - RYK Antibody AP7677a
    Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody (Cat. #AP7677a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).
  • IHC-P - RYK Antibody AP7677a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - RYK Antibody AP7677a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RYK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P34925
Other Accession Q01887
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67507 Da
Antigen Region 160-190 aa
Additional Information
Gene ID 6259
Other Names Tyrosine-protein kinase RYK, RYK, JTK5A
Target/Specificity This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from human RYK.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRYK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RYK
Synonyms JTK5A
Function May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Cellular Location Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm. Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
Tissue Location Observed in all the tissues examined.
Research Areas
Citations ( 0 )

Background

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.

References

Trivier, E., et al., J. Biol. Chem. 277(25):23037-23043 (2002). Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999). Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996). Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993). Stacker, S.A., et al., Oncogene 8(5):1347-1356 (1993).

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$ 295.00
$ 99.00
Cat# AP7677a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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