|Application ||WB, IHC-P, E|
|Other Accession||Q4QQV2, P97465, Q5EA84|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||52392 Da|
|Antigen Region||9-39 aa|
|Other Names||Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62, DOK1|
|Target/Specificity||This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human DOK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DOK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.|
|Cellular Location||Isoform 1: Cytoplasm. Nucleus.|
|Tissue Location||Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer|
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Provided below are standard protocols that you may find useful for product applications.
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
Liang, X., et al., J. Biol. Chem. 277(16):13732-13738 (2002).
Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002).
Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000).
Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000).
Nelms, K., et al., Genomics 53(2):243-245 (1998).
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