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DOK1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DOK1 Antibody (N-term) AP7690a
    Western blot analysis of anti-DOK1 Pab (Cat. #AP7690a) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • WB - DOK1 Antibody (N-term) AP7690a
    Western blot analysis of DOK1 (arrow) using DOK1 Antibody (N-term) (Cat.#AP7690a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK1 gene (Lane 2).
  • IHC-P - DOK1 Antibody (N-term) AP7690a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99704
Other Accession Q4QQV2, P97465, Q5EA84
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52392 Da
Antigen Region 9-39 aa
Additional Information
Gene ID 1796
Other Names Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62, DOK1
Target/Specificity This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human DOK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDOK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DOK1
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Cellular Location Isoform 1: Cytoplasm. Nucleus.
Tissue Location Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer
Research Areas
Citations (0)

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DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.


Liang, X., et al., J. Biol. Chem. 277(16):13732-13738 (2002).
Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002).
Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000).
Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000).
Nelms, K., et al., Genomics 53(2):243-245 (1998).

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$ 295.00
$ 99.00
Cat# AP7690a
(40 western blots)
Availability: In Stock
Bulk Size
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