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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRIB1 Antibody (C-term)   

TRIB1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TRIB1 Antibody (C-term) AP7726b
    Anti-TRIB1 Antibody C-term at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TRIB1 Antibody (C-term) AP7726b
    Anti-TRIB1 Antibody C-term at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TRIB1 Antibody (C-term) AP7726b
    Western blot analysis of anti-TRIB1 Pab (AP7726b) in T47D cell line lysate (35ug/lane). TRIB1(arrow) was detected using the purified Pab.
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96RU8
Other Accession Q8K4K4
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41009 Da
Antigen Region 341-370 aa
Additional Information
Gene ID 10221
Other Names Tribbles homolog 1, TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1, TRIB1 (HGNC:16891)
Target/Specificity This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIB1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIB1 (HGNC:16891)
Function Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.
Tissue Location Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow
Research Areas
Citations ( 0 )

Background

TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.

References

Kiss-Toth, E., et al., J. Biol. Chem. 279(41):42703-42708 (2004).
Wu, M., et al., J. Biol. Chem. 278(29):27072-27079 (2003).
Wilkin, F., et al., Eur. J. Biochem. 248(3):660-668 (1997).

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$ 295.00
$ 99.00
Cat# AP7726b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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