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PUM2 Antibody (S182)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PUM2 Antibody (S182) AP7734d
    Western blot analysis of PUM2 (arrow) using PUM2 Antibody (S182) (Cat.#AP7734d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).
  • IHC-P - PUM2 Antibody (S182) AP7734d
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PUM2 Antibody (S182) AP7734d
    Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TB72
Other Accession Q80U58
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 114216 Da
Antigen Region 159-189 aa
Additional Information
Gene ID 23369
Other Names Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2
Target/Specificity This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from human PUM2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPUM2 Antibody (S182) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PUM2
Synonyms KIAA0235, PUMH2
Function Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, called Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). May regulate DCUN1D3 mRNA levels (PubMed:25349211). Capable of deadenylation-dependent and -independent modes of repression. May support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Cellular Location Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
Tissue Location Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen Expressed in all fetal tissues tested
Research Areas
Citations (0)

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PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.


Kusz,K., Mol. Reprod. Dev. 74 (6), 795-799 (2007)
Spik,A., Reprod Biol 6 SUPPL 1, 37-42 (2006)
Moore,F.L., Proc. Natl. Acad. Sci. U.S.A. 100 (2), 538-543 (2003)

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$ 295.00
$ 99.00
Cat# AP7734d
(40 western blots)
Availability: In Stock
Bulk Size
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