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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NRAS Antibody (N-term)   

NRAS Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NRAS Antibody (N-term) AP7745A
    Western blot analysis of anti-NRAS Antibody (N-term)(Cat.#AP7745a) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab.
  • WB - NRAS Antibody (N-term) AP7745A
    Western blot analysis of NRAS (arrow) using rabbit polyclonal NRAS Antibody (N-term) (Cat.#AP7745a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).
  • FC - NRAS Antibody (N-term) AP7745A
    NRAS Antibody (N-term) (Cat. #AP7745a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - NRAS Antibody (N-term) AP7745A
    Confocal immunofluorescent analysis of NRAS Antibody (N-term)(Cat#AP7745a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IF, E
Primary Accession P01111
Other Accession Q04970, Q2MJK3, P08556, Q5F352
Reactivity Human
Predicted Chicken, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21229 Da
Antigen Region 72-101 aa
Additional Information
Gene ID 4893
Other Names GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1
Target/Specificity This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the N-terminal region of human NRAS.
Dilution WB~~1:1000
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRAS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRAS
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus
Research Areas
Citations (0)

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Background

NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

References

Smalley,K.S., Cancer Res. 68 (14), 5743-5752 (2008)
Banerji,U., Mol. Cancer Ther. 7 (4), 737-739 (2008)

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$ 295.00
$ 99.00
Cat# AP7745A
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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