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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HRAS Antibody (Center)   

HRAS Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HRAS Antibody (Center) AP7764C
    Western blot analysis of lysate from 293T cell line, using HRAS Antibody (Center)(Cat. #AP7764c). AP7764c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - HRAS Antibody (Center) AP7764C
    Western blot analysis of anti-HRAS Antibody (Center) (Cat.#AP7764c) in Jurkat cell line lysates (35ug/lane). HRAS (arrow) was detected using the purified Pab.
  • IHC-P - HRAS Antibody (Center) AP7764C
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - HRAS Antibody (Center) AP7764C
    Confocal immunofluorescent analysis of HRAS Antibody (Center)(Cat#AP7764c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01112
Other Accession P20171, Q61411
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21298 Da
Antigen Region 104-128 aa
Additional Information
Gene ID 3265
Other Names GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1
Target/Specificity This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-128 amino acids from the Central region of human HRAS.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHRAS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
Tissue Location Widely expressed.
Research Areas
Citations ( 0 )


HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. These proteins function in signal transduction pathways. They can bind GTP and GDP, and they have intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in the HRAS gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.


Winter-Vann,A.M., Proc. Natl. Acad. Sci. U.S.A. 100 (11), 6529-6534 (2003)
Coats,S.G., Biochemistry 38 (39), 12926-12934 (1999)
Sakai,E., Int. J. Cancer 52 (6), 867-872 (1992)

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$ 295.00
$ 99.00
Cat# AP7764C
(40 western blots)
Availability: In Stock
Bulk Size
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