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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   B-RAF Antibody (Center)   

B-RAF Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • IF - B-RAF Antibody (Center) AP7810C
    Fluorescent image of C2C12 cell stained with BRAF Antibody (Center)(Cat#AP7810c).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).BRAF immunoreactivity is localized to Cytoplasm significantly.
  • WB - B-RAF Antibody (Center) AP7810C
    Western blot analysis of BRAF Antibody (Center) polyclonal antibody(Cat.#AP7810c)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
  • IHC-P - B-RAF Antibody (Center) AP7810C
    Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center)(Cat.#AP7810c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - B-RAF Antibody (Center) AP7810C
    B-RAF Antibody (Center) (Cat. #AP7810c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15056
Other Accession P28028, Q04982
Reactivity Human
Predicted Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 84437 Da
Antigen Region 424-453 aa
Additional Information
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from the Central region of human B-RAF.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB-RAF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas
Citations (0)

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BRAF, a mebmer of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.


Loewe, R., et al., J. Invest. Dermatol. 123(4):733-736 (2004).
Yamaguchi, T., et al., J. Biol. Chem. 279(39):40419-40430 (2004).
Frattini, M., et al., Oncogene 23(44):7436-7440 (2004).
Tsavachidou, D., et al., Cancer Res. 64(16):5556-5559 (2004).
Gear, H., et al., Invest. Ophthalmol. Vis. Sci. 45(8):2484-2488 (2004).

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Cat# AP7810C
(40 western blots)
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