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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RIPK2 Antibody (C-term)   

RIPK2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RIPK2 Antibody (C-term) AP7818B
    Anti-RIPK2 Antibody (D474) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RIPK2 Antibody (C-term) AP7818B
    Western blot analysis of anti-RIPK2 Pab (Cat. #AP7818b) in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • WB - RIPK2 Antibody (C-term) AP7818B
    Western blot analysis of anti-RIPK2 Pab (Cat. #AP7818b) in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.
  • WB - RIPK2 Antibody (C-term) AP7818B
    Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474) (Cat.#AP7818b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.
  • IHC-P - RIPK2 Antibody (C-term) AP7818B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - RIPK2 Antibody (C-term) AP7818B
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIPK2 Antibody (C-term) (Cat.#AP7818b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43353
Other Accession NP_003812
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61195 Da
Antigen Region 458-488 aa
Additional Information
Gene ID 8767
Other Names Receptor-interacting serine/threonine-protein kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, Tyrosine-protein kinase RIPK2, RIPK2, CARDIAK, RICK, RIP2
Target/Specificity This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human RIPK2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRIPK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RIPK2
Function Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Cellular Location Cytoplasm.
Tissue Location Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node
Research Areas
Citations (0)

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RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.


Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Stehlik, C., et al., J. Biol. Chem. 278(34):31941-31949 (2003).
Chen, Y.R., et al., Biochemistry 42(20):6310-6320 (2003).
Munz, B., et al., Mol. Cell. Biol. 22(16):5879-5886 (2002).
Chin, A.I., et al., Nature 416(6877):190-194 (2002).

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$ 295.00
$ 99.00
Cat# AP7818B
(40 western blots)
Availability: In Stock
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