|Application ||WB, IHC-P, E|
|Calculated MW||61195 Da|
|Antigen Region||458-488 aa|
|Other Names||Receptor-interacting serine/threonine-protein kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, Tyrosine-protein kinase RIPK2, RIPK2, CARDIAK, RICK, RIP2|
|Target/Specificity||This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human RIPK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RIPK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CARDIAK, RICK, RIP2|
|Function||Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.|
|Tissue Location||Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node|
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Provided below are standard protocols that you may find useful for product applications.
RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Stehlik, C., et al., J. Biol. Chem. 278(34):31941-31949 (2003).
Chen, Y.R., et al., Biochemistry 42(20):6310-6320 (2003).
Munz, B., et al., Mol. Cell. Biol. 22(16):5879-5886 (2002).
Chin, A.I., et al., Nature 416(6877):190-194 (2002).
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