|Application ||WB, IHC-P, E|
|Calculated MW||101116 Da|
|Antigen Region||872-899 aa|
|Other Names||Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS|
|Target/Specificity||This MARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 872-899 amino acids from the C-terminal region of human MARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.|
|Cellular Location||Cytoplasm, cytosol|
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Provided below are standard protocols that you may find useful for product applications.
Methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)
Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)
Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)
Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)
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