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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AAK1 Antibody (C-term)   

AAK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AAK1 Antibody (C-term) AP7861B
    Western blot analysis of anti-AAK1 Antibody (C-term) (Cat.#AP7861b) in HepG2 cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q2M2I8
Other Accession P0C1X8, F1SPM8, Q3UHJ0, F1MH24
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 103885 Da
Antigen Region 790-819 aa
Additional Information
Gene ID 22848
Other Names AP2-associated protein kinase 1, Adaptor-associated kinase 1, AAK1, KIAA1048
Target/Specificity This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 790-819 amino acids from the C-terminal region of human AAK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAAK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AAK1
Synonyms KIAA1048
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).
Tissue Location Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain
Research Areas
Citations (0)

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Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.


Henderson,D.M., Mol. Biol. Cell 18 (7), 2698-2706 (2007)
Takahashi,T., Cancer Res. 66 (24), 11932-11937 (2006)
Schmid,E.M., PLoS Biol. 4 (9), E262 (2006)

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$ 295.00
$ 99.00
Cat# AP7861B
(40 western blots)
Availability: In Stock
Bulk Size
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