CYP4Z1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q86W10 |
Other Accession | Q8N1L4 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 59086 Da |
Antigen Region | 89-118 aa |
Gene ID | 199974 |
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Other Names | Cytochrome P450 4Z1, CYPIVZ1, CYP4Z1 |
Target/Specificity | This CYP4Z1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the N-terminal region of human CYP4Z1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CYP4Z1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CYP4Z1 {ECO:0000303|PubMed:19090726, ECO:0000312|HGNC:HGNC:20583} |
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Function | A cytochrome P450 monooxygenase that catalyzes the in-chain oxidation of fatty acids (PubMed:19090726, PubMed:29018033). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates lauric and myristic acids predominantly at the omega-4 and omega-2 positions, respectively (PubMed:19090726, PubMed:29018033). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Displays an absolute stereoselectivity in the epoxidation of arachidonic acid producing the 14(S),15(R)-epoxyeicosatrienoic acid (EET) enantiomer (PubMed:29018033). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:19090726, PubMed:29018033). |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Microsome membrane; Single-pass type II membrane protein |
Tissue Location | Preferentially detected in breast carcinoma tissue and mammary gland, whereas only marginal expression is found in all other tested tissues. |
Provided below are standard protocols that you may find useful for product applications.
Background
CYP4Z1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
References
Savas,U., Arch. Biochem. Biophys. 436 (2), 377-385 (2005)
Rieger,M.A., Cancer Res. 64 (7), 2357-2364 (2004)
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