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AIK Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7900a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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AIK Antibody (N-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | O14965 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 45809 Da |
AIK Antibody (N-term) - Additional info | |
| Gene ID 6790 | |
| Other Names AURKA; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A | |
| Target/Specificity This AIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AIK. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AIK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AIK Antibody (N-term) - Protein Information | |
| Name AURKA | |
| Function Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53 Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis | |
| Cellular Location Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase. Moves to the midbody during both telophase and cytokinesis. Associates with both the pericentriolar material (PCM) and centrioles | |
| Tissue Location Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines | |
AIK Antibody (N-term) - Related products
AP6862a: AIK Antibody (N-term)
AP6863b: AURKA Antibody (C-term)
AP7002a: Aurora-A Antibody (N-term E107)
AP7002b: Aurora-A Antibody (N-term S89)
AP7002c: Aurora-A Antibody (C-term)
AP7900a: AIK Antibody (N-term)
RI10498: AURKA predesign siRNA
DC11922: Human AURKA cDNA Clone
LY10088a: AURKA Over-expression Lysate
BP6862a: AIK Antibody (N-term) Blocking Peptide
BP6863b: AURKA Antibody (C-term) Blocking Peptide
BP7002a: Aurora-A Antibody (N-term E107) Blocking Peptide
BP7002b: Aurora-A Antibody (N-term S89) Blocking Peptide
BP7002c: Aurora-A Antibody (C-term) Blocking Peptide
BP7900a: AIK Antibody (N-term) Blocking Peptide
AT4091a: STK6 Antibody (monoclonal) (M01)
AIK Antibody (N-term) - Application data
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Western blot analysis of AIK (arrow) using rabbit polyclonal hAIK-H105 (Cat. #AP7900a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AIK antibody (N-term) (Cat.#AP7900a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AIK Antibody (N-term) - Research Areas
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BACKGROUND
Aurora A (AIK) plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.
REFERENCES
Kimura, M., et al., J. Biol. Chem. 272(21):13766-13771 (1997).
