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GARS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GARS Antibody (C-term) AP7952b
    All lanes : Anti-GARS Antibody (C-term) at 1:2000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HT-1080 whole cell lysate Lane 5: C6 whole cell lysate Lane 6: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41250
Other Accession NP_002038
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 83166 Da
Antigen Region 706-739 aa
Additional Information
Gene ID 2617
Other Names Glycine--tRNA ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS
Target/Specificity This GARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-739 amino acids from the C-terminal region of human GARS.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Cellular Location Cytoplasm. Mitochondrion. Cell projection, axon Note=Associated with granules in cultured neuron cells
Tissue Location Widely expressed, including brain and spinal cord.
Citations (0)

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GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. The protein has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.


Shiba K., Schimmel P.J. Biol. Chem. 269:30049-30055(1994) Antonellis A., Ellsworth R.E.Am. J. Hum. Genet. 72:1293-1299(2003)

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$ 295.00
$ 99.00
Cat# AP7952b
(40 western blots)
Availability: In Stock
Bulk Size
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