|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||83166 Da|
|Antigen Region||706-739 aa|
|Other Names||Glycine--tRNA ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS|
|Target/Specificity||This GARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-739 amino acids from the C-terminal region of human GARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.|
|Cellular Location||Cytoplasm. Mitochondrion. Cell projection, axon Note=Associated with granules in cultured neuron cells|
|Tissue Location||Widely expressed, including brain and spinal cord.|
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Provided below are standard protocols that you may find useful for product applications.
GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. The protein has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
Shiba K., Schimmel P.J. Biol. Chem. 269:30049-30055(1994) Antonellis A., Ellsworth R.E.Am. J. Hum. Genet. 72:1293-1299(2003)
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