|Application ||WB, IHC-P, E|
|Calculated MW||79686 Da|
|Antigen Region||659-690 aa|
|Other Names||Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-V, HUNK, MAKV|
|Target/Specificity||This HUNK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 659-690 amino acids from the C-terminal region of human HUNK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HUNK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Gardner, H.P., et al., Genomics 63(1):46-59 (2000).
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