|Application ||WB, IHC-P, E|
|Calculated MW||112135 Da|
|Antigen Region||692-721 aa|
|Other Names||Serine/threonine-protein kinase 10, Lymphocyte-oriented kinase, STK10, LOK|
|Target/Specificity||This LOK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692-721 amino acids from the C-terminal region of human LOK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LOK Antibody (C-term E707) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.|
|Cellular Location||Cell membrane; Peripheral membrane protein|
|Tissue Location||Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway.
Kuramochi, S., et al., Immunogenetics 49(5):369-375 (1999).
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