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ARH (LDLRAP1) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARH (LDLRAP1) Antibody (N-term) AP8013a
    Western blot analysis of LDLRAP1 Antibody (N-term) (Cat. #AP8013a) in K562 cell line lysates (35ug/lane). LDLRAP1 (arrow) was detected using the purified Pab.
  • IHC-P - ARH (LDLRAP1) Antibody (N-term) AP8013a
    Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with LDLRAP1 antibody (N-term) (Cat.#AP8013a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q5SW96
Other Accession Q8C142, D3ZAR1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33885 Da
Antigen Region 11-42 aa
Additional Information
Gene ID 26119
Other Names Low density lipoprotein receptor adapter protein 1, Autosomal recessive hypercholesterolemia protein, LDLRAP1, ARH
Target/Specificity This ARH (LDLRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-42 amino acids from the N-terminal region of human ARH (LDLRAP1).
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARH (LDLRAP1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LDLRAP1 (HGNC:18640)
Function Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.
Cellular Location Cytoplasm.
Tissue Location Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes
Research Areas
Citations (0)

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Background

LDLRAP1 is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. This adapter protein is required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). LDLRAP1 may be required for LDL binding and internalization but not for receptor clustering in coated pits. This protein may facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits, and may also be involved in the internalization of other LDLR family members. Mutations in the LDLRAP1 gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.

References

Maurer,M.E., J. Cell. Sci. 119 (PT 20), 4235-4246 (2006)
Keyel,P.A., Mol. Biol. Cell 17 (10), 4300-4317 (2006)

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$ 295.00
$ 99.00
Cat# AP8013a
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Quantity:
(40 western blots)
Availability: In Stock
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