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PI3KR1 Antibody (N-term L11)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, FC, IHC-P-Leica, E |
|---|---|
| Primary Accession | P27986 |
| Other Accession | Q63787, P26450, P23727 |
| Reactivity | Human, Rat |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 83598 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 5295 |
|---|---|
| Other Names | Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1 |
| Target/Specificity | This PI3KR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI3KR1. |
| Dilution | IF~~1:25 WB~~1:2000 IHC-P-Leica~~1:500 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PI3KR1 Antibody (N-term L11) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIK3R1 |
|---|---|
| Synonyms | GRB1 |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). |
| Tissue Location | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level) |
Provided below are standard protocols that you may find useful for product applications.
Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
References
Kobayashi, H., et al., J. Biol. Chem. 279(8):6371-6379 (2004).
Liu, H., et al., J. Cell Biol. 164(4):603-612 (2004).
Sun, M., et al., J. Biol. Chem. 278(44):42992-43000 (2003).
Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
Lee, H.Y., et al., J. Biol. Chem. 278(26):23630-23638 (2003).
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