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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PI3KR1 Antibody (N-term L11)   

PI3KR1 Antibody (N-term L11)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PI3KR1 Antibody (N-term L11) AP8023d
    All lanes : Anti-PI3KR1 Antibody (N-term L11) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PI3KR1 Antibody (N-term L11) AP8023d
    Anti-PI3KR1 Antibody (N-term L11) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PI3KR1 Antibody (N-term L11) AP8023d
    Western blot analysis of anti-PIK3R1 Antibody (N-term L11) Pab (Cat.#AP8023d) in CEM cell line lysates (35ug/lane). PIK3R1(arrow) was detected using the purified Pab.
  • WB - PI3KR1 Antibody (N-term L11) AP8023d
    Western blot analysis of anti-PIK3R1 Antibody (N-term L11) Pab (Cat.#AP8023d) in Hela cell line lysates (35ug/lane). PIK3R1(arrow) was detected using the purified Pab.
  • WB - PI3KR1 Antibody (N-term L11) AP8023d
    Western blot analysis of anti-PI3KR1 Antibody (N-term L11) Pab (Cat.#AP8023d) in 293 cell line lysates transiently transfected with the PIK3R1 gene (3ug/lane). PI3KR1(arrow) was detected using the purified Pab.
  • IHC-P - PI3KR1 Antibody (N-term L11) AP8023d
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1 Antibody (N-term)(Cat.#AP8023d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P27986
Other Accession Q63787, P26450, P23727
Reactivity Human, Rat
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 83598 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 5295
Other Names Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1
Target/Specificity This PI3KR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI3KR1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI3KR1 Antibody (N-term L11) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3R1
Synonyms GRB1
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
Tissue Location Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level)
Research Areas
Citations ( 0 )

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

References

Kobayashi, H., et al., J. Biol. Chem. 279(8):6371-6379 (2004).
Liu, H., et al., J. Cell Biol. 164(4):603-612 (2004).
Sun, M., et al., J. Biol. Chem. 278(44):42992-43000 (2003).
Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
Lee, H.Y., et al., J. Biol. Chem. 278(26):23630-23638 (2003).

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$ 295.00
$ 99.00
Cat# AP8023d
Size:
Quantity:
(40 western blots)
Availability: In Stock
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