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PIP5K2B Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P78356 |
| Other Accession | NP_003550 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 47378 Da |
| Antigen Region | 286-317 aa |
| Gene ID | 8396 |
|---|---|
| Other Names | Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-kinase isoform 2-beta, PIP4K2B, PIP5K2B |
| Target/Specificity | This PIP5K2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-317 amino acids from the C-terminal region of human PIP5K2B. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PIP5K2B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIP4K2B (HGNC:8998) |
|---|---|
| Synonyms | PIP5K2B |
| Function | Participates in the biosynthesis of phosphatidylinositol 4,5- bisphosphate (PubMed:9038203, PubMed:26774281). Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration (PubMed:26774281). Its GTP-sensing activity is critical for metabolic adaptation (PubMed:26774281). PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3 (PubMed:31091439). |
| Cellular Location | Endoplasmic reticulum membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm Note=Associated with the plasma membrane and the endoplasmic reticulum |
| Tissue Location | Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver. |
Provided below are standard protocols that you may find useful for product applications.
Background
PIP5K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. It is a member of the phosphatidylinositol-4-phosphate 5-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor.
References
Rao, V.D., et al., Cell 94(6):829-839 (1998).
Castellino, A.M., et al., J. Biol. Chem. 272(9):5861-5870 (1997).
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