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NEK4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NEK4 Antibody (C-term) AP8076b
    All lanes : Anti-hNEK4-E808 at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - NEK4 Antibody (C-term) AP8076b
    All lanes : Anti-hNEK4-E808 at 1:2000 dilution Lane 1: Y79 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - NEK4 Antibody (C-term) AP8076b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
All lanes : Anti-hNEK4-E808 at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P51957
Other Accession NP_003148
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 94597 Da
Antigen Region 793-824 aa
Additional Information
Gene ID 6787
Other Names Serine/threonine-protein kinase Nek4, Never in mitosis A-related kinase 4, NimA-related protein kinase 4, Serine/threonine-protein kinase 2, Serine/threonine-protein kinase NRK2, NEK4, STK2
Target/Specificity This NEK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-824 amino acids from the C-terminal region of human NEK4.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEK4
Synonyms STK2
Function Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage.
Cellular Location Cell projection, cilium. Cytoplasm
Tissue Location Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, testis, retina, liver, kidney, lung and placenta. Present in most primary carcinomas
Research Areas
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$ 192.50
Cat# AP8076b
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