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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NEK9 Antibody (C-term)   

NEK9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NEK9 Antibody (C-term) AP8079b
    The anti-NEK9 Pab (Cat. #AP8079b) is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).
  • IHC-P - NEK9 Antibody (C-term) AP8079b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TD19
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 107168 Da
Antigen Region 846-877 aa
Additional Information
Gene ID 91754
Other Names Serine/threonine-protein kinase Nek9, Nercc1 kinase, Never in mitosis A-related kinase 9, NimA-related protein kinase 9, NimA-related kinase 8, Nek8, NEK9, KIAA1995, NEK8, NERCC
Target/Specificity This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 846-877 amino acids from the C-terminal region of human NEK9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEK9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEK9
Synonyms KIAA1995, NEK8, NERCC
Function Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Cellular Location Cytoplasm. Nucleus
Tissue Location Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts
Research Areas
Citations (0)

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NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2. NEK9 is activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents. This protein is not cell-cycle regulated but activity is higher in G0-arrested cells. NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP. Interaction is also noted with NEK6 and NEK7 family members. This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts. Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.


Tan,B.C. et al. J. Biol. Chem. 279 (10), 9321-9330 (2004)
Belham,C. et al. J. Biol. Chem. 278 (37), 34897-34909 (2003)
Roig, J. et al. Genes Dev. 16(13):1640-1658 (2002).
Holland, P.M. et al. J. Biol. Chem. 277(18):16229-16240 (2002).
Strausberg, R.L. et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

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$ 295.00
$ 99.00
Cat# AP8079b
(40 western blots)
Availability: In Stock
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