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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PCK2 Antibody (N-term)   

PCK2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PCK2 Antibody (N-term) AP8094a
    Western blot analysis of anti-PCK2 Antibody (N-term) (Cat.#AP8094a) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.
  • IHC-P - PCK2 Antibody (N-term) AP8094a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - PCK2 Antibody (N-term) AP8094a
    Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) (Cat. #AP8094a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.
  • IF - PCK2 Antibody (N-term) AP8094a
    Confocal immunofluorescent analysis of PCK2 Antibody (N-term)(Cat#AP8094a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16822
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70730 Da
Antigen Region 24-54 aa
Additional Information
Gene ID 5106
Other Names Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, PCK2, PEPCK2
Target/Specificity This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCK2
Synonyms PEPCK2
Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.


Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Modaressi, S., et al., Biochem. J. 333 (Pt 2), 359-366 (1998).
Modaressi, S., et al., Biochem. J. 315 (Pt 3), 807-814 (1996).

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$ 295.00
$ 99.00
Cat# AP8094a
(40 western blots)
Availability: In Stock
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