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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PRPK Antibody (Center)   

PRPK Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PRPK Antibody (Center) AP8097c
    The anti-PRPK Pab (Cat. #AP8097c) is used in Western blot to detect PRPK in mouse thymus tissue lysate.
  • IHC-P - PRPK Antibody (Center) AP8097c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - PRPK Antibody (Center) AP8097c
    PRPK Antibody (V235) (Cat. #AP8097c) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the PRPK antibody detected the PRPK protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96S44
Other Accession Q99PW4
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28160 Da
Antigen Region 220-249 aa
Additional Information
Gene ID 112858
Other Names TP53-regulating kinase, Atypical serine/threonine protein kinase TP53RK, EKC/KEOPS complex subunit TP53RK, 36--, Nori-2, p53-related protein kinase, TP53RK, C20orf64, PRPK
Target/Specificity This PRPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human PRPK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRPK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TP53RK
Synonyms C20orf64, PRPK
Function Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis. Weakly expressed in heart kidney and spleen
Research Areas
Citations (0)

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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.


Miyoshi, A., et al., Biochem. Biophys. Res. Commun. 303(2):399-405 (2003).
Abe, Y., et al., J. Biol. Chem. 276(47):44003-44011 (2001).

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$ 295.00
$ 99.00
Cat# AP8097c
(40 western blots)
Availability: In Stock
Bulk Size
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