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UCK Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P30085 |
| Other Accession | Q4KM73, Q29561, Q9DBP5, Q2KIW9 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 22222 Da |
| Antigen Region | 18-48 aa |
| Gene ID | 51727 |
|---|---|
| Other Names | UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 27414 {ECO:0000255|HAMAP-Rule:MF_03172}, Deoxycytidylate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, CK {ECO:0000255|HAMAP-Rule:MF_03172}, dCMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, Nucleoside-diphosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 2746 {ECO:0000255|HAMAP-Rule:MF_03172}, Uridine monophosphate/cytidine monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMPK {ECO:0000255|HAMAP-Rule:MF_03172}, CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172} |
| Target/Specificity | This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human UCK. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UCK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172} |
|---|---|
| Function | Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity. |
| Cellular Location | Nucleus {ECO:0000255|HAMAP-Rule:MF_03172, ECO:0000269|PubMed:10462544, ECO:0000269|PubMed:11912132}. Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03172, ECO:0000269|PubMed:10462544, ECO:0000269|PubMed:11912132}. Note=Predominantly cytoplasmic, less than 15% nuclear. |
| Tissue Location | Ubiquitously expressed. |
Provided below are standard protocols that you may find useful for product applications.
Background
UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Hu, R.M., et al., Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548 (2000).
Hughes, G.J., et al., Electrophoresis 14(11):1216-1222 (1993).
Hochstrasser, D.F., et al., Electrophoresis 13(12):992-1001 (1992).
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