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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PFKL Antibody (C-term L684)   

PFKL Antibody (C-term L684)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PFKL Antibody (C-term L684) AP8136B
    The anti-PFKL Pab (Cat. #AP8136b) is used in Western blot to detect PFKL in HepG2 cell lysate.
  • IHC-P - PFKL Antibody (C-term L684) AP8136B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17858
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85018 Da
Antigen Region 669-699 aa
Additional Information
Gene ID 5211
Other Names ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-L, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, PFK-B, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKL
Target/Specificity This PFKL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-699 amino acids from the C-terminal region of human PFKL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPFKL Antibody (C-term L684) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas
Citations ( 0 )


Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.


Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Hattori, M., et al., Nature 405(6784):311-319 (2000).
Elson, A., et al., Genomics 7(1):47-56 (1990).
Levanon, D., et al., DNA 8(10):733-743 (1989).

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$ 295.00
$ 99.00
Cat# AP8136B
(40 western blots)
Availability: In Stock
Bulk Size
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