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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AK1 Antibody (N-term)   

AK1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AK1 Antibody (N-term) AP8160a
    Western blot analysis of anti-AK1 Pab (Cat. #AP8160a) in Jurkat cell line lysate (35ug/lane). AK1(arrow) was detected using the purified Pab.
  • WB - AK1 Antibody (N-term) AP8160a
    Western blot analysis of AK1 (arrow) using rabbit polyclonal AK1 Antibody (S45) (Cat. #AP8160a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AK1 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00568
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21635 Da
Antigen Region 30-59 aa
Additional Information
Gene ID 203
Other Names Adenylate kinase isoenzyme 1 {ECO:0000255|HAMAP-Rule:MF_03171}, AK 1 {ECO:0000255|HAMAP-Rule:MF_03171}, 2743 {ECO:0000255|HAMAP-Rule:MF_03171}, 2746 {ECO:0000255|HAMAP-Rule:MF_03171}, ATP-AMP transphosphorylase 1 {ECO:0000255|HAMAP-Rule:MF_03171}, ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03171}, Adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03171}, Myokinase {ECO:0000255|HAMAP-Rule:MF_03171}, AK1 {ECO:0000255|HAMAP-Rule:MF_03171}
Target/Specificity This AK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human AK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AK1 {ECO:0000255|HAMAP-Rule:MF_03171}
Function Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.


Corrons, J.L., et al., Blood 102(1):353-356 (2003).
Toren, A., et al., Br. J. Haematol. 87(2):376-380 (1994).
Zuffardi, O., et al., Hum. Genet. 82(1):17-19 (1989).
Matsuura, S., et al., J. Biol. Chem. 264(17):10148-10155 (1989).
Miwa, S., et al., Am. J. Hematol. 14(4):325-333 (1983).

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$ 295.00
$ 99.00
Cat# AP8160a
(40 western blots)
Availability: In Stock
Bulk Size
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