|Application ||WB, IF, E|
|Calculated MW||39298 Da|
|Antigen Region||46-76 aa|
|Other Names||Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, MKP-1, Protein-tyrosine phosphatase CL100, DUSP1, CL100, MKP1, PTPN10, VH1|
|Target/Specificity||This DUSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of human DUSP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Precautions||DUSP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CL100, MKP1, PTPN10, VH1|
|Function||Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.|
|Tissue Location||Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney|
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The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
Wu,W., et al., J. Biol. Chem. 280 (6), 4117-4124 (2005).
Small,G.W., et al., Mol. Pharmacol. 66 (6), 1478-1490 (2004).
Chandrasekharan,U.M., et al., J. Biol. Chem. 279 (45), 46678-46685 (2004).
Mizuno,R., et al., J. Urol. 172 (2), 723-727 (2004).
Vicent,S., et al., Clin. Cancer Res. 10 (11), 3639-3649 (2004).
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