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PLA2G4A Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP8510c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
PLA2G4A Antibody (Center) - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | P47712 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85239 Da |
PLA2G4A Antibody (Center) - Additional info | |
| Gene ID 5321 | |
| Other Names PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase | |
| Target/Specificity This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520~549 amino acids from the Center region of human PLA2G4A. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PLA2G4A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PLA2G4A Antibody (Center) - Protein Information | |
| Name PLA2G4A | |
| Synonyms CPLA2, PLA2G4 | |
| Function Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response | |
| Cellular Location Cytoplasm. Cytoplasmic vesicle. Note=Translocates to membrane vesicles in a calcium-dependent fashion | |
| Tissue Location Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium | |
PLA2G4A Antibody (Center) - Related products
AP8510c: PLA2G4A Antibody (Center)
RI14041: PLA2G4A predesign siRNA
PLA2G4A Antibody (Center) - Application data
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Western blot analysis of PLA2G4A Antibody (Center) (Cat. #AP8510c) in NCI-H460 cell line lysates (35ug/lane). PLA2G4A (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of PLA2G4A Antibody (Center)(Cat. #AP8510c) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PLA2G4A Antibody (Center) - Research Areas
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BACKGROUND
PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
REFERENCES
Sharp,J.D.,et.al., J. Biol. Chem. 266 (23), 14850-14853 (1991) Clark,J.D., et.al., Cell 65 (6), 1043-1051 (1991)
