|Application ||WB, IHC-P, IF, FC, E|
|Other Accession||P50393, Q9TT38, P47713, P50392, P49147, O77793|
|Predicted||Chicken, Zebrafish, Mouse, Rabbit, Rat|
|Calculated MW||85239 Da|
|Antigen Region||513-541 aa|
|Other Names||Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4A, CPLA2, PLA2G4|
|Target/Specificity||This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PLA2G4A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.|
|Cellular Location||Cytoplasm. Cytoplasmic vesicle. Note=Translocates to membrane vesicles in a calcium-dependent fashion|
|Tissue Location||Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium|
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Provided below are standard protocols that you may find useful for product applications.
PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
Sharp,J.D.,et.al., J. Biol. Chem. 266 (23), 14850-14853 (1991)
Clark,J.D., et.al., Cell 65 (6), 1043-1051 (1991)
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