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SIL1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SIL1 Antibody (Center) AP8511c
    Western blot analysis of SIL1 Antibody (Center) (Cat. #AP8511c) in mouse kidney tissue lysates (35ug/lane).SIL1 (arrow) was detected using the purified Pab.
  • IHC-P - SIL1 Antibody (Center) AP8511c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SIL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SIL1 Antibody (Center) AP8511c
    Flow cytometric analysis of HepG2 cells using SIL1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H173
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52085 Da
Antigen Region 99-126 aa
Additional Information
Gene ID 64374
Other Names Nucleotide exchange factor SIL1, BiP-associated protein, BAP, SIL1
Target/Specificity This SIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-126 amino acids from the Central region of human SIL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIL1
Function Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
Cellular Location Endoplasmic reticulum lumen
Tissue Location Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
Research Areas
Citations (0)

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SIL1 IS a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein.


Clark,H.F., ET.AL., Genome Res. 13 (10), 2265-2270 (2003)

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Cat# AP8511c
(40 western blots)
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