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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MyD88 Antibody (Center)   

MyD88 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MyD88 Antibody (Center) AP8521C
    All lanes : Anti-MyD88 Antibody (Center) at 1:1000 dilution Lane 1: Raji whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: HT-29 whole cell lysate Lane 6: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MyD88 Antibody (Center) AP8521C
    Western blot analysis of MyD88 Antibody (Center) (Cat. #AP8521c) in mouse lung tissue lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.(2ug/ml)
  • WB - MyD88 Antibody (Center) AP8521C
    Western blot analysis of MyD88 Antibody (Center) (Cat. #AP8521c) in HepG2 cell line lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.
  • IHC-P - MyD88 Antibody (Center) AP8521C
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MyD88 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - MyD88 Antibody (Center) AP8521C
    MyD88 Antibody (Center) (Cat. #AP8521c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 2
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q99836
Other Accession B3Y682
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33233 Da
Antigen Region 136-164 aa
Additional Information
Gene ID 4615
Other Names Myeloid differentiation primary response protein MyD88, MYD88
Target/Specificity This MyD88 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human MyD88.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyD88 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYD88
Function Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.
Cellular Location Cytoplasm
Tissue Location Ubiquitous..
Research Areas
Citations ( 0 )

Background

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. It acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response and increases IL-8 transcription. It may be involved in myeloid differentiation.

References

Bannon,C., et.al., Biochem. J. 423 (1), 119-128 (2009)
Burns,K., et.al., J. Biol. Chem. 273 (20), 12203-12209 (1998)

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$ 295.00
$ 99.00
Cat# AP8521C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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