|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8CCB4, Q5ZLD7|
|Calculated MW||79653 Da|
|Antigen Region||111-140 aa|
|Other Names||Vacuolar protein sorting-associated protein 53 homolog, VPS53|
|Target/Specificity||This VPS53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human VPS53.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VPS53 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:15878329, PubMed:18367545). Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061).|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein Endosome membrane; Peripheral membrane protein. Recycling endosome. Note=Localizes to the trans-Golgi network as part of the GARP complex, while it localizes to recycling endosomes as part of the EARP complex (PubMed:25799061)|
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Provided below are standard protocols that you may find useful for product applications.
VPS53 is a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi.
Ko,J.K., et.al., J. Cell. Sci. 120 (PT 16), 2912-2923 (2007)
Zhu,J.D., et.al., Cell Res. 16 (9), 780-796 (2006)
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