|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8CCB4, Q5ZLD7|
|Calculated MW||79653 Da|
|Antigen Region||111-140 aa|
|Other Names||Vacuolar protein sorting-associated protein 53 homolog, VPS53|
|Target/Specificity||This VPS53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human VPS53.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VPS53 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:15878329, PubMed:18367545). Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061).|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein Endosome membrane; Peripheral membrane protein. Recycling endosome. Note=Localizes to the trans-Golgi network as part of the GARP complex, while it localizes to recycling endosomes as part of the EARP complex (PubMed:25799061)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
VPS53 is a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi.
Ko,J.K., et.al., J. Cell. Sci. 120 (PT 16), 2912-2923 (2007)
Zhu,J.D., et.al., Cell Res. 16 (9), 780-796 (2006)
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