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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VPS53 Antibody (N-term)   

VPS53 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - VPS53 Antibody (N-term) AP8546a
    Western blot analysis of VPS53 Antibody (N-term) (Cat. #AP8546a) in MCF-7 cell line lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
  • WB - VPS53 Antibody (N-term) AP8546a
    Western blot analysis of VPS53 Antibody (N-term) (Cat. #AP8546a) in mouse brain tissue lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
  • IHC-P - VPS53 Antibody (N-term) AP8546a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with VPS53 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - VPS53 Antibody (N-term) AP8546a
    Flow cytometric analysis of MDA-MB468 cells using VPS53 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5VIR6
Other Accession Q8CCB4, Q5ZLD7
Reactivity Human, Mouse
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79653 Da
Antigen Region 111-140 aa
Additional Information
Gene ID 55275
Other Names Vacuolar protein sorting-associated protein 53 homolog, VPS53
Target/Specificity This VPS53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human VPS53.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS53 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS53
Function May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
Cellular Location Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
Research Areas
Citations (0)

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VPS53 is a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi.


Ko,J.K.,, J. Cell. Sci. 120 (PT 16), 2912-2923 (2007)
Zhu,J.D.,, Cell Res. 16 (9), 780-796 (2006)

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$ 295.00
$ 99.00
Cat# AP8546a
(40 western blots)
Availability: In Stock
Bulk Size
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