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USF2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - USF2 Antibody (Center) AP8585c
    Western blot analysis of USF2 Antibody (Center) (Cat. #AP8585c) in mouse cerebellum tissue lysates (35ug/lane).USF2 (arrow) was detected using the purified Pab.
  • IHC-P - USF2 Antibody (Center) AP8585c
    USF2 Antibody (Center) (Cat. #AP8585c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the USF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q15853
Other Accession Q63665, Q64705
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 218-246 aa
Additional Information
Other Names Upstream stimulatory factor 2, Class B basic helix-loop-helix protein 12, bHLHb12, FOS-interacting protein, FIP, Major late transcription factor 2, Upstream transcription factor 2, USF2, BHLHB12
Target/Specificity This USF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human USF2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USF2
Synonyms BHLHB12
Function Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Cellular Location Nucleus.
Tissue Location Ubiquitous. EMBL; X90824; CAA62339.1; -; mRNA EMBL; X90825; CAA62340.1; -; mRNA EMBL; X90826; CAA62341.1; -; mRNA EMBL; Y07661; CAA68942.1; -; Genomic_DNA EMBL; AY147880; AAN63092.1; -; mRNA EMBL; CR536504; CAG38742.1; -; mRNA EMBL; AD000684; AAB51179.1; -; Genomic_DNA EMBL; BC049821; AAH49821.1; -; mRNA EMBL; S50537; AAB24368.1; -; mRNA EMBL; M77476; -; NOT_ANNOTATED_CDS; mRNA CCDS; CCDS12452.1; -. [Q15853-1] CCDS; CCDS12453.1; -. [Q15853-3] CCDS; CCDS82329.1; -. [Q15853-4] PIR; I54074; I54074 RefSeq; NP_001308079.1; NM_001321150.1. [Q15853-4] RefSeq; NP_003358.1; NM_003367.3. [Q15853-1] RefSeq; NP_997174.1; NM_207291.2. [Q15853-3] UniGene; Hs.454534; - ProteinModelPortal; Q15853; - SMR; Q15853; - BioGrid; 113238; 28 ComplexPortal; CPX-3079; USF1-USF2 upstream stimulatory factor complex ComplexPortal; CPX-3083; USF2 upstream stimulatory factor complex CORUM; Q15853; - IntAct; Q15853; 29 MINT; Q15853; - STRING; 9606.ENSP00000222305; - iPTMnet; Q15853; - PhosphoSitePlus; Q15853; - BioMuta; USF2; - DMDM; 2833271; - EPD; Q15853; - MaxQB; Q15853; - PaxDb; Q15853; - PeptideAtlas; Q15853; - PRIDE; Q15853; - ProteomicsDB; 60794; - ProteomicsDB; 60795; -. [Q15853-2] ProteomicsDB; 60796; -. [Q15853-3] Ensembl; ENST00000222305; ENSP00000222305; ENSG00000105698. [Q15853-1] Ensembl; ENST00000343550; ENSP00000340633; ENSG00000105698. [Q15853-3] Ensembl; ENST00000379134; ENSP00000368429; ENSG00000105698. [Q15853-4] Ensembl; ENST00000595068; ENSP00000471099; ENSG00000105698. [Q15853-2] GeneID; 7392; - KEGG; hsa:7392; - UCSC; uc002nyq.2; human. [Q15853-1] CTD; 7392; - DisGeNET; 7392; - EuPathDB; HostDB:ENSG00000105698.15; - GeneCards; USF2; - HGNC; HGNC:12594; USF2 HPA; HPA029764; - HPA; HPA046567; - MIM; 600390; gene neXtProt; NX_Q15853; - OpenTargets; ENSG00000105698; - PharmGKB; PA37224; - eggNOG; ENOG410INSC; Eukaryota eggNOG; ENOG4111DV1; LUCA GeneTree; ENSGT00390000004498; - HOGENOM; HOG000294174; - HOVERGEN; HBG004346; - InParanoid; Q15853; - KO; K09106; - PhylomeDB; Q15853; - TreeFam; TF323338; - Reactome; R-HSA-9018519; Estrogen-dependent gene expression SignaLink; Q15853; - SIGNOR; Q15853; - ChiTaRS; USF2; human GeneWiki; USF2; - GenomeRNAi; 7392; - PRO; PR:Q15853; - Proteomes; UP000005640; Chromosome 19 Bgee; ENSG00000105698; - CleanEx; HS_USF2; - ExpressionAtlas; Q15853; baseline and differential Genevisible; Q15853; HS GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005634; C:nucleus; IDA:BHF-UCL GO; GO:0043425; F:bHLH transcription factor binding; IPI:BHF-UCL GO; GO:0003700; F:DNA binding transcription factor activity; TAS:ProtInc GO; GO:0046982; F:protein heterodimerization activity; IPI:BHF-UCL GO; GO:0042803; F:protein homodimerization activity; IPI:BHF-UCL GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0043565; F:sequence-specific DNA binding; IDA:BHF-UCL GO; GO:0003705; F:transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; IMP:BHF-UCL GO; GO:0007595; P:lactation; IEA:Ensembl GO; GO:0019086; P:late viral transcription; IC:BHF-UCL GO; GO:0055088; P:lipid homeostasis; ISS:BHF-UCL GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:BHF-UCL GO; GO:0000432; P:positive regulation of transcription from RNA polymerase II promoter by glucose; IMP:BHF-UCL GO; GO:0000430; P:regulation of transcription from RNA polymerase II promoter by glucose; IC:BHF-UCL GO; GO:0006366; P:transcription by RNA polymerase II; IMP:BHF-UCL CDD; cd00083; HLH; 1 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 1: Evidence at protein level; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Reference proteome; Transcription; Transcription regulation CHAIN 1 346 Upstream stimulatory factor 2 /FTId=PRO_0000127500 DOMAIN 235 290 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} REGION 307 328 Leucine-zipper COMPBIAS 11 20 Poly-Ala COMPBIAS 245 248 Poly-Arg VAR_SEQ 76 206 Missing (in isoform USF2c) /FTId=VSP_047804 VAR_SEQ 77 143 Missing (in isoform USF2B) /FTId=VSP_002164 VAR_SEQ 275 282 Missing (in isoform USF2A-delta-H) /FTId=VSP_002165 CONFLICT 46 64 QTAVAITSVQQAAFGDHNI -> GGGTSGGRGSGIQTRVQH V (in Ref. 7). CONFLICT 93 100 GDTAGAVS -> EFHSWRRH (in Ref. 8; M77476) CONFLICT 122 122 A -> V (in Ref. 8; M77476) CONFLICT 308 308 Q -> R (in Ref. 4; CAG38742) SEQUENCE 346 AA; 36955 MW; 78CEFE97AC4C10CF CRC64; MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ
Citations (0)

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Background

USF2 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. This protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs.

References

Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Groenen,P.M., et.al., Genomics 38 (2), 141-148 (1996)

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Cat# AP8585c
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