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HPD Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HPD Antibody (N-term) AP8591a
    Anti-HPD Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HPD Antibody (N-term) AP8591a
    Western blot analysis of HPD Antibody (N-term) (Cat. #AP8591a)in mouse liver tissue lysates (35ug/lane). HPD (arrow) was detected using the purified Pab.
  • IHC-P - HPD Antibody (N-term) AP8591a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HPD Antibody (N-term) AP8591a
    Flow cytometric analysis of HepG2 cells using HPD Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P32754
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44934 Da
Antigen Region 92-121 aa
Additional Information
Gene ID 3242
Other Names 4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD
Target/Specificity This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human HPD.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHPD Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HPD
Synonyms PPD
Function Key enzyme in the degradation of tyrosine.
Research Areas
Citations (0)

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Background

HPD is an enzyme in the catabolic pathway of tyrosine. This protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate.

References

Item,C.B., et.al., Mol. Genet. Metab. 91 (4), 379-383 (2007)
Wistow,G., et.al., Mol. Vis. 8, 171-184 (2002)

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$ 295.00
$ 99.00
Cat# AP8591a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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