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CYP4B1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CYP4B1 Antibody (Center) AP8650C
    Western blot analysis of CYP4B1 Antibody (Center) (Cat. #AP8650c) in MDA-MB468 cell line lysates (35ug/lane). CYP4B1 (arrow) was detected using the purified Pab.
  • IHC-P - CYP4B1 Antibody (Center) AP8650C
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CYP4B1 Antibody (Center) (Cat. #AP8650c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - CYP4B1 Antibody (Center) AP8650C
    Confocal immunofluorescent analysis of CYP4B1 Antibody (Center) (Cat. #AP8650c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13584
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58991 Da
Antigen Region 331-362 aa
Additional Information
Gene ID 1580
Other Names Cytochrome P450 4B1, CYPIVB1, Cytochrome P450-HP, CYP4B1
Target/Specificity This CYP4B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-362 amino acids from the Central region of human CYP4B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP4B1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP4B1
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location Detected in the liver and lung (at protein level).
Research Areas
Citations (0)

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CYP4B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined.


McKinnon,R.A.,, Pharmacogenetics 4 (5), 260-270 (1994)
Yokotani,N.,, Eur. J. Biochem. 187 (1), 23-29 (1990)

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Cat# AP8650C
(40 western blots)
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