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TSPAN1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TSPAN1 Antibody (Center) AP8693c
    Western blot analysis of TSPAN1 Antibody (Center) (Cat. #AP8693c) in mouse heart tissue lysates (35ug/lane). TSPAN1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - TSPAN1 Antibody (Center) AP8693c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TSPAN1 Antibody (Center) AP8693c
    TSPAN1 Antibody (Center) (Cat. #AP8693c) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O60635
Other Accession NP_005718.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26301 Da
Antigen Region 140-167 aa
Additional Information
Gene ID 10103
Other Names Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1
Target/Specificity This TSPAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids of human TSPAN1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSPAN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSPAN1
Cellular Location Lysosome membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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Background

TSPAN1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.

References

Xu,D., et.al., FASEB J. 23 (11), 3674-3681 (2009)
Berditchevski,F.et.al., J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)

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$ 365.00
$ 140.00
Cat# AP8693c
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