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HSPA6 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HSPA6 Antibody (C-term) AP8708b
    Western blot analysis of lysate from Jurkat cell line,using HSPA6 Antibody (C-term)(Cat. #AP8708b).AP8708b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
  • IHC-P - HSPA6 Antibody (C-term) AP8708b
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPA6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HSPA6 Antibody (C-term) AP8708b
    HSPA6 Antibody (C-term) (Cat. #AP8708b) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P17066
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 71028 Da
Antigen Region 544-571 aa
Additional Information
Gene ID 3310
Other Names Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B', HSPA6, HSP70B'
Target/Specificity This HSPA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-571 amino acids from the C-terminal region of human HSPA6.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA6
Synonyms HSP70B'
Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).
Research Areas
Citations (0)

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Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

References

Leung,T.K., et.al.,Genomics 12 (1), 74-79 (1992)

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$ 295.00
$ 99.00
Cat# AP8708b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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