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HNF1A Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HNF1A Antibody (Center) AP8717c
    Western blot analysis of HNF1A Antibody (Center) (Cat. #AP8717c) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab.
  • IF - HNF1A Antibody (Center) AP8717c
    Confocal immunofluorescent analysis of HNF1A Antibody (Center)(Cat#AP8717c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20823
Other Accession P15257, P22361
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67356 Da
Antigen Region 177-205 aa
Additional Information
Gene ID 6927
Other Names Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, LFB1, Transcription factor 1, TCF-1, HNF1A, TCF1
Target/Specificity This HNF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the Central region of human HNF1A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHNF1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HNF1A
Synonyms TCF1
Function Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'- GTTAATNATTAAC-3'.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:10966642}
Tissue Location Liver.
Citations (0)

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HNF1A is a transcription factor required for the expression of several liver-specific genes. This protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.


Bach,I.,, Genomics 8 (1), 155-164 (1990)

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Cat# AP8717c
(40 western blots)
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