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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CYP7B1 Antibody (Center)   

CYP7B1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYP7B1 Antibody (Center) AP8786c
    Western blot analysis of CYP7B1 Antibody (Center) (Cat. #AP8786c) in Hela cell line lysates (35ug/lane). CYP7B1 (arrow) was detected using the purified Pab.
  • IHC-P - CYP7B1 Antibody (Center) AP8786c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP7B1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CYP7B1 Antibody (Center) AP8786c
    CYP7B1 Antibody (Center) (Cat. #AP8786c) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - CYP7B1 Antibody (Center) AP8786c
    Confocal immunofluorescent analysis of CYP7B1 Antibody (Center) (Cat. #AP8786c) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, IF, WB, E
Primary Accession O75881
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58256 Da
Antigen Region 252-281 aa
Additional Information
Gene ID 9420
Other Names 25-hydroxycholesterol 7-alpha-hydroxylase, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase, CYP7B1
Target/Specificity This CYP7B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human CYP7B1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP7B1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP7B1
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine
Research Areas
Citations (0)

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Background

CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

References

Schwarz,M., et.al., Curr. Opin. Lipidol. 9 (2), 113-118 (1998)

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$ 295.00
$ 99.00
Cat# AP8786c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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