|Application ||IHC-P, FC, WB, E|
|Calculated MW||56806 Da|
|Antigen Region||250-279 aa|
|Other Names||Lanosterol 14-alpha demethylase, LDM, CYPLI, Cytochrome P450 51A1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450LI, Sterol 14-alpha demethylase, CYP51A1, CYP51|
|Target/Specificity||This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human CYP51A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CYP51A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.|
|Cellular Location||Endoplasmic reticulum membrane. Microsome membrane|
|Tissue Location||Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung|
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Provided below are standard protocols that you may find useful for product applications.
CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.
Matsuura,K., et.al., J. Biol. Chem. 280 (10), 9088-9096 (2005)
Wang,Y.,et.al., J. Biol. Chem. 283 (39), 26332-26339 (2008)
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