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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   GPA33 Antibody (C-term)   

GPA33 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GPA33 Antibody (C-term) AP8888B
    All lanes : Anti-GPA33 Antibody (C-term) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GPA33 Antibody (C-term) AP8888B
    Western blot analysis of GPA33 Antibody (C-term) (Cat. #AP8888b) in K562, 293, MDA-MB231 cell line lysates (35ug/lane). GPA33 (arrow) was detected using the purified Pab.
  • IHC-P - GPA33 Antibody (C-term) AP8888B
    GPA33 Antibody (C-term) (Cat. #AP8888b) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPA33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - GPA33 Antibody (C-term) AP8888B
    GPA33 Antibody (C-term) (Cat. #AP8888b) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99795
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35632 Da
Antigen Region 264-293 aa
Additional Information
Gene ID 10223
Other Names Cell surface A33 antigen, Glycoprotein A33, GPA33
Target/Specificity This GPA33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human GPA33.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPA33 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPA33
Function May play a role in cell-cell recognition and signaling.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in normal gastrointestinal epithelium and in 95% of colon cancers
Research Areas
Citations (0)

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GPA33 is a cell surface antigen that is expressed in greater than 95% of human colon cancers. It may play a role in cell-cell recognition and signaling.


Moritz,R.L.,, J Chromatogr A 798 (1-2), 91-101 (1998)

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$ 295.00
$ 99.00
Cat# AP8888B
(40 western blots)
Availability: In Stock
Bulk Size
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