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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   B4GalT1 Antibody (C-term)   

B4GalT1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - B4GalT1 Antibody (C-term) AP8892b
    Western blot analysis of B4GalT1 Antibody (C-term) (Cat. #AP8892b) in HL-60 cell line lysates (35ug/lane). B4GALT1 (arrow) was detected using the purified Pab.
  • FC - B4GalT1 Antibody (C-term) AP8892b
    B4GalT1 Antibody (C-term) (Cat. #AP8892b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15291
Other Accession P15535, P08037
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43920 Da
Antigen Region 329-358 aa
Additional Information
Gene ID 2683
Other Names Beta-1, 4-galactosyltransferase 1, Beta-1, 4-GalTase 1, Beta4Gal-T1, b4Gal-T1, 241-, UDP-Gal:beta-GlcNAc beta-1, 4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1, 4-galactosyltransferase 1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1, 4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1, 4-galactosyltransferase, 241-, Processed beta-1, 4-galactosyltransferase 1, B4GALT1, GGTB2
Target/Specificity This B4GalT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-358 amino acids from the C-terminal region of human B4GalT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB4GalT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B4GALT1
Synonyms GGTB2
Function The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Cellular Location Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium {ECO:0000250|UniProtKB:P15535}. Note=Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903). B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity). {ECO:0000250|UniProtKB:P15535, ECO:0000269|PubMed:1714903} Processed beta-1,4-galactosyltransferase 1: Secreted. Note=Soluble form found in body fluids
Tissue Location Ubiquitously expressed, but at very low levels in fetal and adult brain
Research Areas
Citations (0)

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B4GalT1 is an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing.


Mengle-Gaw,L.,, Biochem. Biophys. Res. Commun. 176 (3), 1269-1276 (1991)

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Cat# AP8892b
(40 western blots)
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