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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   HLA-G Antibody (Center)   

HLA-G Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HLA-G Antibody (Center) AP8941c
    All lanes : Anti-HLA-G Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HLA-G Antibody (Center) AP8941c
    Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody (Center))(Cat. #AP8941c). AP8941c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • WB - HLA-G Antibody (Center) AP8941c
    Western blot analysis of HLA-G Antibody (Center) (Cat. #AP8941c) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
  • WB - HLA-G Antibody (Center) AP8941c
    Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) (Cat. #AP8941c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
  • IHC-P - HLA-G Antibody (Center) AP8941c
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HLA-G Antibody (Center) AP8941c
    HLA-G Antibody (Center) (Cat. #AP8941c) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17693
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38224 Da
Antigen Region 62-89 aa
Additional Information
Gene ID 3135
Other Names HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, MHC class I antigen G, HLA-G, HLA-60, HLAG
Target/Specificity This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-G Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-G
Synonyms HLA-6.0, HLAG
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in trophoblasts.
Research Areas
Citations ( 0 )


HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.


Walpole,N.G.,, J. Mol. Biol. 397 (2), 467-480 (2010)
Tian,W.,, Tissue Antigens 75 (3), 227-234 (2010)

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$ 295.00
$ 99.00
Cat# AP8941c
(40 western blots)
Availability: In Stock
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