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CLIP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CLIP1 Antibody (N-term) AP8950a
    Anti-CLIP1 Antibody (N-term) at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 162 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CLIP1 Antibody (N-term) AP8950a
    CLIP1 Antibody (N-term) (Cat. #AP8950a) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - CLIP1 Antibody (N-term) AP8950a
    CLIP1 Antibody (N-term) (Cat. #AP8950a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30622
Other Accession O55156, Q9Z0H8, Q9UDT6, Q922J3, O42184
Reactivity Human
Predicted Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 162246 Da
Antigen Region 228-254 aa
Additional Information
Gene ID 6249
Other Names CAP-Gly domain-containing linker protein 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, CLIP-170, Reed-Sternberg intermediate filament-associated protein, Restin, CLIP1, CYLN1, RSN
Target/Specificity This CLIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the N-terminal region of human CLIP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLIP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLIP1
Synonyms CYLN1, RSN
Function Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Note=Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles
Tissue Location Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
Research Areas
Citations (0)

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CLIP1 may be a intermediate filament associated protein that links endocytic vesicles to microtubules.


Yang,X.,, J. Biol. Chem. 284 (42), 28775-28782 (2009)
Meunier,B.,, Eur. J. Cell Biol. 88 (2), 91-102 (2009)

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$ 295.00
$ 99.00
Cat# AP8950a
(40 western blots)
Availability: In Stock
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