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KIF11 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KIF11 Antibody (N-term) AP9097A
    Anti-KIF11 Antibody (N-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 119 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - KIF11 Antibody (N-term) AP9097A
    Western blot analysis of KIF11 Antibody (N-term) (Cat. #AP9097a) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.
  • IHC-P - KIF11 Antibody (N-term) AP9097A
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - KIF11 Antibody (N-term) AP9097A
    KIF11 Antibody (N-term) (Cat.#AP9097a) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52732
Reactivity Human, Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 119159 Da
Antigen Region 103-130 aa
Additional Information
Gene ID 3832
Other Names Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5
Target/Specificity This KIF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human KIF11.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKIF11 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIF11
Synonyms EG5, KNSL1, TRIP5
Function Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, spindle pole
Research Areas
Citations (0)

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KIF11 belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.


Tcherniuk,S.,, Biochem. Pharmacol. 79 (6), 864-872 (2010)
Parke,C.L.,, J. Biol. Chem. 285 (8), 5859-5867 (2010)

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$ 295.00
$ 99.00
Cat# AP9097A
(40 western blots)
Availability: In Stock
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