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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CFLAR Antibody (Center)   

CFLAR Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CFLAR Antibody (Center) AP9123c
    Western blot analysis of CFLAR Antibody (Center) (Cat. #AP9123c) in HepG2 cell line lysates (35ug/lane). CFLAR (arrow) was detected using the purified Pab.
  • IHC-P - CFLAR Antibody (Center) AP9123c
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CFLAR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CFLAR Antibody (Center) AP9123c
    CFLAR Antibody (Center) (Cat. #AP9123c) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O15519
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55344 Da
Antigen Region 145-174 aa
Additional Information
Gene ID 8837
Other Names CASP8 and FADD-like apoptosis regulator, Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, CASP8 and FADD-like apoptosis regulator subunit p43, CASP8 and FADD-like apoptosis regulator subunit p12, CFLAR, CASH, CASP8AP1, CLARP, MRIT
Target/Specificity This CFLAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human CFLAR.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCFLAR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CFLAR
Synonyms CASH, CASP8AP1, CLARP, MRIT
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Tissue Location Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle
Research Areas
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Background

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. It lacks enzymatic (caspase) activity.

References

Kim,T.W., et.al., Science 277 (5324), 373-376 (1997)

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$ 295.00
$ 99.00
Cat# AP9123c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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