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EIF4A2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EIF4A2 Antibody (C-term) AP9190B
    Anti-EIF4A2 Antibody (C-term) at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EIF4A2 Antibody (C-term) AP9190B
    Western blot analysis of EIF4A2 Antibody (C-term) (Cat. #AP9190b) in K562 cell line lysates (35ug/lane). EIF4A2 (arrow) was detected using the purified Pab.
  • IHC-P - EIF4A2 Antibody (C-term) AP9190B
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EIF4A2 Antibody (C-term) AP9190B
    EIF4A2 Antibody (C-term) (Cat. #AP9190b) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - EIF4A2 Antibody (C-term) AP9190B
    Western blot analysis of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody (C-term) (Cat. #AP9190b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14240
Other Accession Q5RKI1, P10630, Q4R4Y9, Q8JFP1, Q3SZ65, P29562, P60843, Q4R8K5, P60842, Q3SZ54
Reactivity Human
Predicted Bovine, Monkey, Mouse, Rabbit, Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46402 Da
Antigen Region 333-360 aa
Additional Information
Gene ID 1974
Other Names Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA helicase eIF4A-2, EIF4A2, DDX2B, EIF4F
Target/Specificity This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4A2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4A2
Synonyms DDX2B, EIF4F
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Citations (0)

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Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).


Martins-de-Souza,D.,, J Neural Transm 116 (3), 275-289 (2009)
Lin,C.J.,, Cancer Res. 68 (13), 5326-5334 (2008)

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$ 295.00
$ 99.00
Cat# AP9190B
(40 western blots)
Availability: In Stock
Bulk Size
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