- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||P35435, Q91VR2, Q4R5B0, P05631|
|Predicted||Bovine, Monkey, Mouse, Rat|
|Calculated MW||32996 Da|
|Antigen Region||40-67 aa|
|Other Names||ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, ATP5C1, ATP5C, ATP5CL1|
|Target/Specificity||This ATP5C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-67 amino acids from the N-terminal region of human ATP5C1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP5C1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.|
|Cellular Location||Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein|
|Tissue Location||Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta|
Provided below are standard protocols that you may find useful for product applications.
ATP5C1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3.
Wheeler,H.E., et.al, PLoS Genet. 5 (10), E1000685 (2009)
Wang,L., et.al, Cancer Epidemiol. Biomarkers Prev. 17 (12), 3558-3566 (2008)
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