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ABCF1 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP9278b 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP9278b-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
  • Specification
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  • Backgrounds

ABCF1 Antibody (C-term) - Product info

ApplicationWB, IHC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionQ8NE71
ReactivityHuman, Mouse
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW95926 Da

ABCF1 Antibody (C-term) - Additional info

Gene ID 23
Other Names
ABCF1; ABC50; ATP-binding cassette sub-family F member 1; ATP-binding cassette 50; TNF-alpha-stimulated ABC protein
Target/Specificity
This ABCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 694~724 amino acids from the C-terminal region of human ABCF1.
Dilution
WB~~1:100~500
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ABCF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

ABCF1 Antibody (C-term) - Protein Information

Name ABCF1
Synonyms ABC50
Function
Isoform 2 is required for efficient Cap- and IRES- mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis
Cellular Location
Isoform 2: Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope
Tissue Location
Ubiquitous.

ABCF1 Antibody (C-term) - Related products

AP9278b: ABCF1 Antibody (C-term)

RI10027: ABCF1 predesign siRNA

BP9278b: ABCF1 Antibody (C-term) Blocking Peptide

ABCF1 Antibody (C-term) - Application data

  • Western blot analysis of ABCF1 Antibody (C-term)(Cat. #AP9278b) in NCI-H460 cell line lysates (35ug/lane). ABCF1 (arrow) was detected using the purified Pab.

  • Western blot analysis of ABCF1 Antibody (C-term)(Cat. #AP9278b) in mouse NIH-3T3 cell line lysates (35ug/lane). ABCF1 (arrow) was detected using the purified Pab.

  • ABCF1 Antibody (C-term) (Cat. #AP9278b) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ABCF1 Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

The protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process.

REFERENCES

Barcellos,L.F., et.al., PLoS Genet. 5 (10), E1000696 (2009) Paytubi,S., et.al., J. Biol. Chem. 284 (36), 24061-24073 (2009) Saito,A., et.al., J. Hum. Genet. 54 (6), 317-323 (2009)