Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GFPT2 Antibody (Center)   

GFPT2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GFPT2 Antibody (Center) AP9282c
    Western blot analysis of GFPT2 Antibody (Center) (Cat. #AP9282c) in A2058, K562 cell line lysates (35ug/lane). GFPT2 (arrow) was detected using the purified Pab.
  • IHC-P - GFPT2 Antibody (Center) AP9282c
    GFPT2 Antibody (Center) (Cat. #AP9282c) IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFPT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - GFPT2 Antibody (Center) AP9282c
    GFPT2 Antibody (Center) (Cat. #AP9282c) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O94808
Other Accession Q4KMC4, Q9Z2Z9, Q08DQ2
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 175-201 aa
Additional Information
Other Names Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2, D-fructose-6-phosphate amidotransferase 2, Glutamine:fructose-6-phosphate amidotransferase 2, GFAT 2, GFAT2, Hexosephosphate aminotransferase 2, GFPT2
Target/Specificity This GFPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human GFPT2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFPT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GFPT2
Function Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
Tissue Location Highest levels of expression in heart, placenta, and spinal cord EMBL; AB016789; BAA74731.1; -; mRNA EMBL; BT009818; AAP88820.1; -; mRNA EMBL; BC000012; AAH00012.1; -; mRNA CCDS; CCDS43411.1; - RefSeq; NP_005101.1; NM_005110.3 UniGene; Hs.696497; - ProteinModelPortal; O94808; - BioGrid; 115270; 36 IntAct; O94808; 2 STRING; 9606.ENSP00000253778; - DrugBank; DB00130; L-Glutamine MEROPS; C44.972; - iPTMnet; O94808; - PhosphoSitePlus; O94808; - BioMuta; GFPT2; - EPD; O94808; - MaxQB; O94808; - PaxDb; O94808; - PeptideAtlas; O94808; - PRIDE; O94808; - ProteomicsDB; 50448; - DNASU; 9945; - Ensembl; ENST00000253778; ENSP00000253778; ENSG00000131459 GeneID; 9945; - KEGG; hsa:9945; - UCSC; uc003mlw.2; human CTD; 9945; - DisGeNET; 9945; - EuPathDB; HostDB:ENSG00000131459.12; - GeneCards; GFPT2; - HGNC; HGNC:4242; GFPT2 HPA; HPA056892; - HPA; HPA059910; - MIM; 603865; gene neXtProt; NX_O94808; - OpenTargets; ENSG00000131459; - PharmGKB; PA28652; - eggNOG; KOG1268; Eukaryota eggNOG; COG0449; LUCA GeneTree; ENSGT00390000010049; - HOGENOM; HOG000258898; - HOVERGEN; HBG051724; - InParanoid; O94808; - KO; K00820; - OMA; RLDSSTC; - OrthoDB; EOG091G02N5; - PhylomeDB; O94808; - TreeFam; TF300864; - Reactome; R-HSA-446210; Synthesis of UDP-N-acetyl-glucosamine UniPathway; UPA00113; UER00528 ChiTaRS; GFPT2; human GenomeRNAi; 9945; - PRO; PR:O94808; - Proteomes; UP000005640; Chromosome 5 Bgee; ENSG00000131459; - CleanEx; HS_GFPT2; - ExpressionAtlas; O94808; baseline and differential Genevisible; O94808; HS GO; GO:0005829; C:cytosol; TAS:Reactome GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IBA:GO_Central GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEA:Ensembl GO; GO:0006112; P:energy reserve metabolic process; TAS:ProtInc GO; GO:0006002; P:fructose 6-phosphate metabolic process; TAS:ProtInc GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central GO; GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; TAS:Reactome GO; GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IBA:GO_Central CDD; cd05008; SIS_GlmS_GlmD_1; 1 CDD; cd05009; SIS_GlmS_GlmD_2; 1 InterPro; IPR017932; GATase_2_dom InterPro; IPR035466; GlmS/AgaS_SIS InterPro; IPR035490; GlmS/FrlB_SIS InterPro; IPR005855; GlmS_trans InterPro; IPR029055; Ntn_hydrolases_N InterPro; IPR001347; SIS Pfam; PF01380; SIS; 2 SUPFAM; SSF56235; SSF56235; 2 TIGRFAMs; TIGR01135; glmS; 1 PROSITE; PS51278; GATASE_TYPE_2; 1 PROSITE; PS51464; SIS; 2 1: Evidence at protein level; Aminotransferase; Complete proteome; Glutamine amidotransferase; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase INIT_MET 1 1 Removed. CHAIN 2 682 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 /FTId=PRO_0000135283 DOMAIN 2 288 Glutamine amidotransferase type-2 {ECO:0000255|PROSITE-ProRule:PRU00609} DOMAIN 360 499 SIS 1. {ECO:0000255|PROSITE- ProRule:PRU00797} DOMAIN 531 672 SIS 2. {ECO:0000255|PROSITE- ProRule:PRU00797} REGION 377 378 Substrate-binding {ECO:0000250|UniProtKB:Q06210} REGION 422 424 Substrate-binding {ECO:0000250|UniProtKB:Q06210} ACT_SITE 2 2 For GATase activity {ECO:0000250|UniProtKB:P14742} BINDING 427 427 Substrate {ECO:0000250|UniProtKB:Q06210} BINDING 578 578 Substrate {ECO:0000250|UniProtKB:Q06210} MOD_RES 244 244 Phosphoserine VARIANT 471 471 I -> V (in dbSNP:rs2303007) {ECO:0000269|Ref.2} /FTId=VAR_013311 SEQUENCE 682 AA; 76931 MW; A090D75526F3192B CRC64; MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

Background

GFPT2 controls the flux of glucose into the hexosamine pathway. This protein most likely involved in regulating the availability of precursors for N-and O-linked glycosylation of proteins.

References

Edwards,T.L., et.al., Ann. Hum. Genet. 74 (2), 97-109 (2010)
Prasad,P., et.al., BMC Med. Genet. 11, 52 (2010)
Srinivasan,V., et.al., Clin. Biochem. 40 (13-14), 952-957 (2007)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 295.00
$ 99.00
Cat# AP9282c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
“Crown Flash”  50 % off on 2,500+ Crown Antibodies. PromoCode:<span class=text-red> FLASH50
Terms & Conditions