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PNOC Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PNOC Antibody (Center) AP9313c
    All lanes : Anti-PNOC Antibody (Center) at 1:4000 dilution Lane 1: human brain lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PNOC Antibody (Center) AP9313c
    Western blot analysis of PNOC Antibody (Center) (Cat. #AP9313c) in HepG2 cell line lysates (35ug/lane). PNOC (arrow) was detected using the purified Pab.
  • IHC-P - PNOC Antibody (Center) AP9313c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PNOC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13519
Other Accession Q62923, Q64387, O62647
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20295 Da
Antigen Region 126-151 aa
Additional Information
Gene ID 5368
Other Names Prepronociceptin, Neuropeptide 1, Nociceptin, Orphanin FQ, PPNOC, Neuropeptide 2, PNOC, OFQ
Target/Specificity This PNOC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-151 amino acids from the Central region of human PNOC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPNOC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms OFQ
Function Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity).
Cellular Location Secreted.
Tissue Location Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation
Research Areas
Citations (0)

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PNOC is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. This protein may be involved in neuronal differentiation and development


Kuzmin,A.,, Brain Res. 1305 SUPPL, S80-S85 (2009)
Rocha,L.,, Neurobiol. Dis. 35 (3), 466-473 (2009)
de Krom,M.,, Biol. Psychiatry 65 (7), 625-630 (2009)

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Cat# AP9313c
(40 western blots)
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